Scientific Literature



Article History: Micro- and nanoscale surface modifications have been the focus of multiple studies in the pursuit of accelerating 24bone apposition or osseointegration at the implant surface. Here, we evaluated histological and nanomechanical 25properties, and gene expression, for a microblasted surface presenting nanometer-scale texture within a 26micrometer-scale texture (MB) (Ossean Surface, Intra-Lock International, Boca Raton, FL) versus a dual-acid 27etched surface presenting texture at the micrometer-scale only (AA), in a rodent femur model for 1, 2, 4, and 8 weeks in vivo.


Following animal sacrifice, samples were evaluated in terms of histomorphometry, biomechanical29 properties through nanoindentation, and gene expression by real-time quantitative reverse transcription 30polymerase chain reaction analysis. Although the histomorphometric, and gene expression analysis results 31were not significantly different between MB and AA at 4 and 8 weeks, significant differences were seen at 1 32and 2 weeks.


The expression of the genes encoding collagen type I (COL-1), and osteopontin (OPN) was significantly33 higher for MB than for AA at 1 week, indicating up-regulated osteoprogenitor and osteoblast differentiation34. At 2 weeks, significantly up-regulated expression of the genes for COL-1, runt-related transcription factor 2 35(RUNX-2), osterix, and osteocalcin (OCN) indicated progressive mineralization in newly formed bone.


The nanomechanical36 properties tested by the nanoindentation presented significantly higher-rank hardness and elastic 37modulus for the MB compared to AA at all time points tested. In conclusion, the nanotopographical featured 38surfaces presented an overall higher host-to-implant response compared to the microtextured only surfaces.


39The statistical differences observed in some of the osteogenic gene expression between the two groups may 40shed some insight into the role of surface texture and its extent in the observed bone healing mechanisms. 4142 © 2014 Published by Elsevier Inc.

Nanometer-scale features onmicrometer-scale surface texturing: A bone histological, gene expression, and nano mechanical study



Bone, Issue 65, Aug. 2014. Bone (2014),


Surface: 1

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