Platelet-Rich Fibrin (L-PRF)

Centrifuge System Process

Platelet-rich fibrin (L-PRF) is an autogenous matrix derived from the concentration of the patient’s blood platelets. A simplified chairside procedure results in the production of a fibrin membrane that stimulates bone and soft tissue growth. It is rich in leukocytes and vascular endothelial growth factor (VEGF).

L-PRF also initiates the sustained release of platelet-derived growth factor (PDGF), a protein that plays a significant role in angiogenesis; transforming growth factor (TGF beta), a protein that stimulates tissue growth; and thrombospondin 1, an adhesive glycoprotein that mediates cell-to-cell matrix interactions and angiogenesis. The presence of these proteins induces rapid healing, especially during the critical first days after placement.

The main property of this autologus biomaterial is the growth factor’s slow release by the L-PRF during more than seven days. This slow release is only possible with the L-PRF membrane, not with PRP or PRGF. The L-PRF “Box” is a special tray used to automatically produce membranes of constant thickness and size. The L-PRF exudate produced by this “Box” contains a great quantity of proteins specialized in increasing cell attachment to biomaterials and titanium.

Clinically, the membrane displays excellent working properties. It is resilient, strong and pliable, making it easy to manipulate, cut and suture. L-PRF is also exceptionally stable at room temperature, enabling ample working time. The membrane is easily fabricated (L-PRF “Box”) and is very similar to a natural, post-surgical fibrin network. The biological and biomimetic qualities of this membrane not only encourages efficient cell migration and proliferation, but also eliminates the need for biochemical additives or the use of anticoagulants.

Formation of the membrane is easily accomplished chairside. Essentially, it is created by following a precise protocol for centrifuging a given quantity of the patient’s blood, decanting, and cutting the membrane to size with a constant thickness.

Top portion is platelet poor plasma, middle is L-PRF, and bottom contains red cells (RBC's)

Tweezers grasp fibrin clot with attached RBC's