Scientific Literature



Background and Objectives: Platelet concentrates for surgical use (Platelet-Rich Plasma PRP or Platelet-rich fibrin PRF) are surgical adjuvants to improve healing and promote tissue regeneration. L-PRF (Leukocyte- and Platelet-Rich Fibrin) is one of the 4 families of platelet concentrates for surgical use and is widely used in oral and maxillofacial regenerative therapies. The objective of this first article was to evaluate the mechanical vibrations appearing during centrifugation in 4 models of commercially available table centrifuges frequently used to produce L-PRF.


Materials and Methods: The 4 different tested centrifuges were the original L-PRF centrifuge (Intra-Spin, Intra-Lock, the only CE and FDA cleared system for the preparation of L-PRF) and 3 other laboratory centrifuges (not CE nor FDA cleared for L-PRF): A-PRF 12 (Advanced PRF, Process), LW - UPD8 (LW Scientific) and Salvin 1310 (Salvin Dental). Each centrifuge was opened for inspection, two accelerometers were installed (one radial, one vertical), and data were collected with a spectrum analyzer. Each centrifuge was tested in 2 configurations (full-load or half load with 9ml blood collection tubes filled with water) and at the following rotational speeds: 1500, 1800, 2100, 2400, 2700, 3000 and 3300 rpm. Extra rotational speeds were used on some centrifuges. One centrifuge (Salvin) had only one available rotational speed (3400 rpm). For each test, the software documented both radial and vertical vibration.


Results: Very significant differences in the level of vibrations at each rotational speed were observed between the 4 tested machines. The original L-PRF centrifuge (Intra-Spin) was by far the most stable machine in all configurations. At the classical speed of production of L- PRF, the level of undesirable vibration on this centrifuge is between 4.5 and 6 times lower than with other centrifuges. Moreover, Intra-Spin always remains under the threshold of resonance, unlike the 3 other tested machines.


Discussion and Conclusion: Each centrifuge has its clear own profile of vibrations depending on the rotational speed, and this may impact significantly the characteristics of the PRP or PRF produced with these devices. This result may reveal a considerable flaw in all the PRP/PRF literature, as this parameter was never considered. It is now necessary to evaluate the impact of the vibration parameter on the architecture and cell content of the L- PRF clots produced with these 4 different machines.


Keywords: Blood platelets, growth factors, leukocytes, platelet-rich plasma, regenerative medicine, wound healing

The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors and fibrin architecture of a Leukocyte- and Platelet-Rich Fibrin (L-PRF) clot and membrane. Part 1: evaluation of the vibration shocks of 4 models of table centrifuges for L-PRF.



POSEIDO, Volume 2, Issue 2, June 2014, Pages 129-39.


Medicine: 3

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